Pei max sigma. 동의어(들): Ethyleneimine polymer solution, PEI.

Pei max sigma Polyethylenimine 'Max' (PEI MAX®) is a powerful, trusted, and cost-effective reagent widely considered as a current gold standard for both in vitro and in vivo transfection. 注文検索 PEI Prime™线性聚乙烯亚胺 suitable for gene delivery; CAS Number: 49553-93-7; Synonyms: PEI; Linear Formula: (C2H5N . C6628. 7. : 9002-98-6. Polymer Composition. Pour the solution into a 500-mL glass cylinder. Incubate the mixture at room temperature for 30 min. Poly(ethyleneimine) solution. Branched PEI can be synthesized by cationic ring-opening polymerization of aziridine. Quick View. ログイン. Because components required Sigma-Aldrich. Poly(ethyleneimine) solution average Mw 1800, 50 wt. Filter-sterilize the solution through a 0. PEI MAX 40K是由Polysciences出品的全合成线性聚乙烯亚胺瞬时转染试剂. HCl)n at Sigma-Aldrich 线性聚乙烯亚胺是一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子物质。工业应用方面，线性聚乙烯亚胺可以调整染料的化学特性并增强染料与固相基质的粘附能力，从而改善负电荷染料的染色效果，常用作黏附增强剂，作用与多聚赖氨酸(poly-lysine)类似。 MCE PEI Transfection Reagent 是一种基于分子量 25 kDa PEI 的 DNA 转染试剂，通过引入可特异性结合 DNA 的功能基因对 PEI 进行改性，显著增强了 PEI 和 DNA 的结合能力，并且降低了细胞毒性。另外，通过功能性优化显著增强了 PEI 的细胞摄取、内涵体逃逸等功能。 聚乙烯亚胺是一种水溶性聚合物，用于过滤冷冻细胞膜的膜润滑剂。小柱中的pei纤维素将从相关化合物中分离环核苷酸，其电解质浓度与在pei纤维素涂层板上进行薄层色谱时所用的电解质浓度相同。 ポリエチレンイミン、線状 average Mn 10,000, PDI ≤1. , GS filter, Millipore) and store the solution at 4°C. Add the PEI-DNA mixture to the suspension cells from Step 2. 22-μm membrane. HCl)n at Sigma-Aldrich Add 10 ml of polyethylenimine (Polymin P, BASF or BRL, molecular-weight range 30,000-40,000) to 70 ml of H 2 O. Sigma-Aldrich brand products are sold through Sigma-Aldrich, Inc. Available for Sale. PEI MAX 也叫线性化聚乙烯亚胺 PEI 40000 （ Polyethylenimine Linear, MW 40000 ）， 是一款基于聚乙烯亚胺（ Polyethylenimine L ， PEI ）的产品，带有高电荷阳离子，非常容易结合带负电荷的 DNA 分子，形成复合物，并转染到 溶解性高：pei 40000较易溶解，可以直接在水中溶解，而pei 25000需要先把水调成弱酸性以 助其溶解，溶解后再用naoh把ph调至中性。 2. 408700. Dilute 495 µl of PEI into 495 µl of pre-warmed medium for 990 µl of PEI (900 µl + 10%) Briefly vortex the mixture and spin to return the solution to the bottom of the tube. Quality Segment. . 製品 アプリケーション サービス 技術資料 サポート. sigmaaldrich. カート 0. Sigma-Aldrich. PEI MAX Linear Polyethylenimine Hydrochloride (MW 40,000), Non GMP, Research Grade Solid PEI MAX 40K (cat# 24765) is a non-GMP research grade solid transfection reagent popular for academic and early stage research. PEI MAX has a high density of protonatable amino groups, with PEI MAX® is a high-efficiency polyethylenimine (PEI) transfection reagent for both DNA and RNA delivery in HEK293, CHO, and other cell lines. PEI Prime™ linear polyethylenimine suitable for gene delivery; CAS Number: 49553-93-7; Synonyms: PEI; Linear Formula: (C2H5N . 线性化聚乙烯亚胺（简称： 线性pei ）分两种分子量25k和40k。 两者 区别: 1) pei 25k转染溶液通常需要几个小时才能制备，pei 25k含有4-1 1%的残余丙酰基，可防止聚合物主链与dna强烈结合。 2) pei max 40k 完全水解(完全去乙酰化的结构意味着每批产品的表现始终如)， 可以在两个小时内转化为即用型溶液。 Request PDF | Transient Mammalian Cell Transfection with Polyethylenimine (PEI) | Standard protein expression systems, such as E. Polyethylenimine, Linear, MW 25000（PEI 25K，PEI 25，000），是一种强大的，值得信赖且非常有效的瞬时转染试剂。 在HEK293和CHO表达系统， 在各种生产规模（从96孔板到100L生物反应器），PEI都提供连续性的高基因表达水平。 PEI F25-LMW is prepared from the commercially available branched 25 kD polyethylenimine (Sigma-Aldrich, St. We strive to provide our customers with a one-stop shop for the entire scientific supplies category. 无残留物：PEI 40000无丙酰基残留，这意味着它更易于与DNA结合，而PEI 25000含有 4-11% 的丙酰基残留，这些残留物可能会阻止聚合物主链与 DNA 产品简介: Polyethylenimine HCl MAX, Linear, Mw 40,000 线性化聚乙烯亚胺盐酸盐MAX（PEI 40，000） 基本描述： Polyethylenimine HCl MAX, Linear, Mw 40,000（PEI MAX 40K，PEI 40，000）， 不含盐形式也称为PEI 22K，是一种强大的，值得信赖且非常有效的瞬时转染试剂。 在HEK293和CHO表达系统，在各种生产规模（从96孔板到100L生物 Polyethylenimine “Max” (PEI MAX) is a highly efficient transfection reagent which is compatible for a wide range of cell lines/types including the most commonly used HEK293 and CHO cells grown in adherent and suspension cultures. Louis, MO, USA) by gel filtration as described . Supplier: Polysciences, Inc. Linear Formula: CH 3 (NHC 2 H 4) n OH·xHCl. PEI Prime Polyethylenimine (PEI) is a hydrophilic cationic polymer widely used as a nonviral nucleotide delivery reagent. United States Globally. linear, average M n 作为线性化聚乙烯亚胺pei25000（pei 25k）的升级产品，操作更简单，且具有连续性的更高表达滴度，优势在于：1）pei 25k转染溶液通常需几个小时来配置，然而，pei 40k在2个小时内即可转化为即用型的溶液；2）pei 25k含4-11%残留的丙酰基基团，该基团阻止聚合物骨架紧密结合到dna。 ease of use, PEI is a popular transfection reagent used at bioreactor scale for transient transfection. PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) (24765) Catalog download. average M n ~60,000 by GPC, average M w ~750,000 by LS, 50 wt. I dissolve 5 mg PEI in 5 mL ultrapure water and vortex, then I heat at 85 PEI MAX® Linear Polyethylenimine Hydrochloride (MW 40,000), Non-GMP, Research Grade Solid PEI MAX 40K (cat# 24765) is a non-GMP research grade solid transfection reagent popular for academic and early stage research. 2 µm filter and stored at 4°C (see PEI Prime™线性聚乙烯亚胺 suitable for gene delivery; CAS Number: 49553-93-7; Synonyms: PEI; Linear Formula: (C2H5N . 6. 4; Synonyms: Linear PEI HCl salt,Poly(ethylene imine hydrochloride),Poly(ethylene imine) HCl salt; Linear Formula: CH3(NHC2H4)nOH·xHCl at Sigma-Aldrich Polyethylenimine “Max” (PEI MAX) is a highly efficient transfection reagent which is compatible for a wide range of cell lines/types including the most commonly used HEK293 and CHO cells grown in adherent and suspension cultures. Key Advantages Superior Performance: High transfection efficiency with low cytotoxicity compared to other reagents on the market, suitable for use in larger concentrations and in sensitive cells. I prepared 1 mg/mL PEI (Polyethylenimine, linear average Mn 5,000, PDI ≤1. 764647. 製品. Suspension HEK-293 and CHO cells were seeded at 1×106 cells/mL in serum free medium and transfected with PEIpro®, PEI MAX and L-PEI 25 kDa (Polysciences, Warrington, PA) resuspended at 1 mg/mL. Polymer Type. Find PEI Max and related products for scientific research at Merck For 50 ml transfection (final volume = 100 ml) you need 450 µg PEI at a concentration of 0. 0 μg/ml, (Sigma) according to the manufacturer’s instructions. PEI MAX是一款高度浓缩的粉剂,性价比高,在HEK293, CHO 和 Sf9系统中可获得很高的转染效率,在重组抗体和病毒载体生产中稳定性高,可靠性强,适用于基础学术研究和药物研发早期阶段. SIGMA-X Easy install Newly designed cosmeticsRotatable HF waveguidesRe-evaluated performanceUpdated column models with HF driversColumn models with mini EVO-Fold waveguides70 V / 100 V Hi-Z Column modelsEASE gll countries. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). It is ideal for gene expression and viral vector Find PEI Max and related products for scientific research at Merck. 22-μm nitrocellulose filter (e. US Headquarters. Orthogonal approaches to product characterization support better understanding of your asset's performance, safety, and the manufacturing processes for Drug Substance (DS) and Drug Product (DP). More relevant content may be added as Sigma-Aldrich. PEI MAX® has a high density of protonatable amino groups, with pei max是一种功能强大，值得信赖且经济实惠的瞬时转染试剂，在hek293和cho大多数表达系统中，pei都能提供稳定的高转染效率。 与传统PEI 25K相比性能更加稳定，而且可以在两个小时内完成整个转染过程，相对于大多数转染试剂 Polyethylenimine hydrochloride linear, average Mn 10,000, PDI ≤1. HCl)n at Sigma-Aldrich Sigma-Aldrich. Each year, more researchers and companies turn to Polysciences PEI to gain a critical edge in their work. HCl)n; find Sigma-Aldrich-919012 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich 转染新宠——PEI MAX （线性PEI MW 40000）更高效的转染试剂，PEI MAX 也叫线性化聚乙-烯亚胺PEI 40000（Polyethylenimine Linear, MW 40000，是一款基于聚乙烯亚胺（Polyethylenimine L，PEI）的产品，是一种分子量为40000的瞬时转染试剂，带有高电荷阳离子，非常容易结合带负电荷的DNA分子，形成复合物，并转染到贴壁 作为线性化聚乙烯亚胺pei25000（pei 25k）的升级产品，操作更简单，且具有连续性的更高表达滴度，优势在于：1）pei 25k转染溶液通常需几个小时来配置，然而，pei 40k在2个小时内即可转化为即用型的溶液；2）pei 25k含4-11%残留的丙酰基基团，该基团阻止 聚合物骨架紧密结合 Find PEI Max and related products for scientific research at Merck Polysciences, Inc. 6 Polyethylenimine “Max” High Potency Linear PEI A nearly fully hydrolyzed linear polyethylenimine with longer contiguous ethyleneimine segments. Polyethylenimine (PEI) is a hydrophilic cationic polymer widely used as a nonviral nucleotide delivery reagent. 5 µg/µl. PEI comes in linear and branched configurations, ranging in molecular weight from 800 to 1,000,000 Da (ref. PEI MAX® has a high density of protonatable amino groups, with Polyethylenimine “Max” (PEI MAX) is a highly efficient transfection reagent which is compatible for a wide range of cell lines/types including the most commonly used HEK293 and CHO cells grown in adherent and suspension cultures. 40000 (PEI-MAX), Linear, Transfection Grade, VWR 75800-188; Transfer the PEI-MAX and recombinant antibody plasmid DNA sample to the biosafety cabinet and incubate for 1 h at room temperature. Filter the PEI solution through a 0. HIGHLIGHTS • Non-GMP, research grade solution • For pre-cGMP pilot studies • Provides seamless transition to 聚乙烯亚胺“max”(pei max) 是一种功能强大、值得信赖且具有成本效益的试剂，被广泛认为是目前体外和体内的金标准转染。pei max 具有高密度的可质子化氨基，每隔三个原子就有一个氨基氮。这赋予了几乎任何 ph 值下的高缓冲能力。 PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. % in H2O; CAS Number: 9002-98-6; Synonyms: Ethyleneimine polymer solution,PEI at Sigma-Aldrich PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) (24765) Polyethylenimine Hydrochloride, Linear (MW 160,000) (25439) Catalog download. Covalently functionalized SWNTs find application in CO 2 absorption and gene delivery. 5uL Plasmid DNA : 1ug 相当 (62. HIGHLIGHTS • Non-GMP, research grade solution • For pre-cGMP pilot studies • Provides seamless transition to 丁香通为您提供Polysciencespolysciences PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride价格等详细信息，产品供应商为北京百奥创新科技有限公司，产品类别为试剂-细胞生物学检测-细胞转染，货号是24765-1X。如需批量采购，还请访问丁香通官网。 PEI MAX 40K（在游离碱中也称PEI 22K）是一种瞬时转染试剂，以功能强大，经济实惠而著称。在HEK293和CHO表达系统中，能在96孔板至100L生物反应器范围内提供始终如一高效的基因表达。 其他： NIST（中国总代理） 、Strem、Wako、Sigma、TCI、Thermo #3_cell #transfection Aim PEIMAXを使って、発現チェックや安定発現細胞の作製のために細胞にプラスミドを導入する Material 培養細胞 (35mm dish) O-MEM : 62. Locations. 8. CAS No. 5. Adjust the final volume to 500 mL with Milli-Q H 2 O. Sigma-Aldrich (10) Supelco (1) 끓는점(°C) 녹는점(°C) 동의어(들): Ethyleneimine polymer solution, PEI. Add 900 µl of PEI/medium mix to the cells. W. Chloroquine PEI cellulose in small columns will separate cyclic nucleotides from related compounds at the same electrolyte concentration that is used for thin layer chromatography on PEI PEI MAX is capable of yielding high efficiency cell lines without compromising cell viability compared to other PEI and liposomal transfection reagents available in the market. ポリ（エチレンイミン） 溶液 ~50% in H2O; CAS Number: 9002-98-6; Synonyms: PEI,エチレンイミンポリマー 溶液 at Sigma-Aldrich Polyethylenimine “Max” (PEI MAX®) は、in vitroおよびin vivo のトランスフェクションにおいて現在のゴールドスタンダードと広く認識されており、かつ、コストパフォーマンスに優れた試薬です。 HEK293 や CHO 細胞をはじめとする多様な細胞株・細胞種に適しており、接着培養・浮遊培養のいずれにも PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. Dilute the appropriate amount of PEI ‘Max,’ as determined in Step 1, into a total volume of 20 ml of Hybridoma SFM (without serum). Serum-free media that support cell growth, transfection and protein expression for PEI mediated transient transfection can better meet regulatory compliance and simplify downstream product purification. 产品简介: Polyethylenimine，Linear，Mw 25，000 线性化聚乙烯亚胺（PEI 25，000） 基本描述： 线性化聚乙烯亚胺PEI 25，000 ，一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子基质。 工业应用上，线性化PEI可改善负电荷 Poly(ethyleneimine) solution average Mn ~60,000 by GPC, average Mw ~750,000 by LS, 50 wt. Feature. Titrate the solution to pH 7. 8. Aquí nos gustaría mostrarte una descripción, pero el sitio web que estás mirando no lo permite. Sigma-Aldrich (1) Polyethylenimine 'Max' (PEI MAX®) is a powerful, trusted, and cost-effective reagent widely considered as a current gold standard for both in vitro and in vivo transfection. PEI MAX is capable of yielding high efficiency cell lines without compromising cell viability compared to other PEI and liposomal transfection reagents available in the market. 2. POLYETHYLENIMINE HCL MAX. The same expression levels were obtained with both PEI variants (data not shown), and since PEI max 40 kDa is easier to handle this variant was chosen for the experiments described in this study. 9 with concentrated HCl, and adjust the final volume to 100 ml with H 2 O. Warrington, PA 18976. 1. PEI cellulose in small columns will separate cyclic nucleotides from related compounds at the same electrolyte concentration that is used for thin layer chromatography on PEI cellulose-coated plates. KEY ADVANTAGES Superior Performance: High transfection efficiency with low cytotoxicity compared to other reagents on the market, suitable for use in larger concentrations and in sensitive cells. Melting Point (°C) Physical Form. 5 uL PEIMAX (1 mg/mL) : 2. 4. The expression efficiency and cost per produced amount of antibody was compared with the PEI Max 40 kDa transfection protocol and the ExpiFectamine Polyethylenimine, branched average Mw ~800 by LS, average Mn ~600 by GPC; CAS Number: 25987-06-8; Synonyms: PEI,ethylenediamine branched; Linear Formula: H(NHCH2CH2)nNH2 at Sigma-Aldrich peiは、水性媒体中の金属ナノ粒子の作製において反応性テンプレートおよび安定化剤として機能します。peiのアミノ基は、水に溶解している金属イオンと配位して、凝集能力を低下させることができます。その結果、さらに小さなナノ粒子が形成されます。 PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. HCl)n at Sigma-Aldrich o PEI stock solution (stored at ‐20 C, Darwin, Bowdish Laboratory) diluted from branched polyethylenimine – makes cells more porous (Sigma, Co#: 408727) PROTOCOL ‐ Preparatory Work: o The day before transfection, plate 1 x 105 cells into 6‐well plates in Among the first cationic polymers to be used in transfection was polyethylenimine (PEI). 181978. The 2-AB labelling reaction was performed at 60 °C for 2 h, 我用PEI（sigma 25kDa）作为转染试剂，但是发现，同样的细胞，同样的N/P 条件下。转染效果一次比一次要差，难道是PEI容易在水性条件下降解？ 我们的这个PEI买的时间比较久了，不知道是不是该买新的了。 一直找不出原因，很是郁闷，请大虾帮忙解答， PEI MAX Linear Polyethylenimine Hydrochloride (MW 40,000), Non GMP, Research Grade Solid PEI MAX 40K (cat# 24765) is a non-GMP research grade solid transfection reagent popular for academic and early stage research. Although N-deacylation reactions are notoriously diffi cult, our new linear polyethylenimine material is 工业应用上，线性化PEI可改善负电荷染料的物理外观，以调整染料的化学特性和增强染料与固相基质的黏附能力，常用作黏附增强剂，作用同多聚赖氨酸（poly-lysine）；科研应用上，线性化PEI能与DNA或其他负电荷生物大分子简单结合，正是这一特性，使得成为一种非常行之有效的载体运输介质（Vector PEI MAX is capable of yielding high efficiency cell lines without compromising cell viability compared to other PEI and liposomal transfection reagents available in the market. Store aliquots of the desired volume at -20°C. US Homes for sale in Canada – Find newest MLS listings for sale, sold, for rent, listing history, open houses, and market stats. coli, often fail to produce folded, monodisperse, or functional PEI Prime™ linear polyethylenimine suitable for gene delivery; CAS Number: 49553-93-7; Synonyms: PEI; Linear Formula: (C2H5N . Polyethylenimine “Max” (PEI MAX®) は、 in vitro および in vivo のトランスフェクションにおいて現在のゴールドスタンダードと広く認識されており、かつ、コストパフォーマンスに優れた試薬です。 HEK293 や CHO 細 Polyethylenimine hydrochloride (Linear PEI HCl salt) | Buy chemicals and reagents online from Sigma Aldrich PEI MAX 也叫 线性化聚乙烯亚胺PEI 40000（Polyethylenimine Linear, MW 40000， 是 一款基于聚乙烯亚胺（Polyethylenimine L，PEI）的产品，是 一种分子量为40000的瞬时转染试剂，带有高电荷阳离子，非常容易结合带负电荷 支化聚乙烯亚胺 average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched; CAS Number: 9002-98-6; Synonyms: 氮丙啶，均聚物,PEI; Linear Formula: H(NHCH2CH2)nNH2 at Sigma-Aldrich Polyethylenimine hydrochloride linear, average Mn 15,000, PDI <1. 919012. pei 25k含有4-11％的残留丙酰基，这可能阻碍聚合物主链与dna的有效结合，所以批间转染效率可能会有比较大的差异，建议一个批次可以多购买，pei 25k稳定性在室温干燥避光下可稳定保存10年以上（虽然有效期标注只有18个月左右）。 2. Add the diluted PEI ‘Max’ to the diluted DNA and mix. 5 uLの O-MEMに懸濁したDNA溶液を用意しておくy-goto 20 聚乙烯亚胺“Max” (PEI MAX) 是一种功能强大、值得信赖且具有成本效益的试剂，被广泛认为是目前体外和体内的金标准转染。PEI MAX 具有高密度的可质子化氨基，每三个原子有一个氨基氮。这赋予了几乎在任何 pH 值下的高缓冲能力。因此，一旦进入内体，PEI MAX 就会破坏液泡并将遗传物质释放到细胞 Polyethylenimine “Max” (PEI MAX) is a highly efficient transfection reagent which is compatible for a wide range of cell lines/types including the most commonly used HEK293 and CHO cells grown in adherent and suspension cultures. Branched PEI can be synthesized by cationic ring-opening polymerization of The cationic polymer polyethylenimine (PEI) has been widely used for nucleic acid delivery due to Multi-paratopic VEGF decoy receptor have superior anti-tumor effects through anti-EGFRs PEI Prime ™ is a cationic polymer designed for high-yield, reproducible and scalable drug and gene delivery. CiteULike; Plasmid DNA and PEI (PEI MAX, PolySciences) were diluted separately in 1/20th of the final culture volume of F17 (final concentrations of DNA and PEI were 1. com and/or on the reverse side of the invoice or packing slip. Polyethylenimine HCl MAX, Linear, Mw 40,000（PEI 40，000；PEI MAX 40K）是热卖产品线性化聚乙烯亚胺PEI25,000（PEI 25K）的升级产品，不同之处在于1）完全水解（去乙酰化）；2）PEI25，000的盐酸盐形式，具更高的水溶特性；3）含更长连续性乙烯亚胺（ethyleneimine）片段，其质子化氮水平比PEI25，000提高11%以上。 Three commercially available linear polyethylenimines (25 kDa LPEI, 40 kDa PEI“Max” and PEIpro™) were compared regarding their potency to transfect serum-free growing and suspension-adapted HEK293 and CHO cells. % in H2O; CAS Number: 9002-98-6; Synonyms: Ethyleneimine polymer solution,PEI at Sigma-Aldrich ポリエチレンイミン、分岐 average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched; CAS Number: 9002-98-6; Synonyms: アジリジン、ホモポリマー,PEI; Linear Formula: H(NHCH2CH2)nNH2 at Sigma-Aldrich Sigma-Aldrich (31) Color. 5; Synonyms: Linear PEI HCl salt,Poly(ethylene imine hydrochloride),Poly(ethylene imine) HCl salt; Linear Formula: CH3(NHC2H4)nOH·xHCl at Sigma-Aldrich 支化聚乙烯亚胺 average Mw ~270,000 by LS; CAS Number: 9002-98-6; Synonyms: PEI; Linear Formula: H(NHCH2CH2)nNH2 at Sigma-Aldrich Valproic acid sodium salt, Sigma Aldrich P4543-10G; Polyethylenimine hydrochloride, M. Purchaser must determine the suitability of the product(s) for their particular use. Chloroquine PEI cellulose in small columns will separate cyclic nucleotides from related compounds at the same electrolyte concentration that is used for thin layer chromatography on PEI 全部 精品仪器 GattaQuant ADS Biotec Sigma-Aldrich Bioclone Nebulabio Glycon Biochem Sterlitech PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) 24765 (1) 1g. Branched PEI can also be used to modify the surface properties of adsorbents. 3, Sigma) using the heating process. 400 Valley Road. % in H 2 O. As a gene carrier, PEI forms a complex with nucleic acids via electrostatic self Polyethylenimine “Max” (PEI MAX) is a powerful, trusted, and cost-effective reagent widely considered as a current gold standard for both in vitro and in vivo transfection. Polyethylenimin -Lösung analytical standard, 50 % (w/v) in H2O; CAS Number: 9002-98-6; Synonyms: Ethylenimin-Polymer -Lösung,PEI at Sigma-Aldrich Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution. It is filter sterilized through a 0. 3; CAS Number: 9002-98-6; Synonyms: PEI; Linear Formula: (CH2CH2NH)n at Sigma-Aldrich コンテンツへスキップ. Please see product information on the Sigma-Aldrich website at www. 1). 2. Additional terms and conditions may apply. HIGHLIGHTS • Non-GMP, research grade solution • For pre-cGMP pilot studies • Provides seamless transition to PEI MAX L-PEI 25K (1 mg/mL) PEIpro® requires less reagent and similar to lower DNA amount compared to other PEIs. 准备 DNA-PEI 复合物： DNA、PEI 试剂和稀释剂在进行以下步骤前需先使其升至室 温。依据表 1 所示，用 Opti-MEMITM（Invitrogen）或其他适合的无蛋白培养基稀释适量 DNA。 用同样的培养基稀释 PEI 试剂。每 1 μg DNA 需用 1-5 μL 线性PEI转染试剂(这个需要 PEI Prime™ linear polyethylenimine suitable for gene delivery; CAS Number: 49553-93-7; Synonyms: PEI; Linear Formula: (C2H5N . g. Synonym(s): Linear PEI HCl salt, Poly(ethylene imine hydrochloride), Poly(ethylene imine) HCl salt. 247651/DUP This product was recently added by customer request, and is available for your convenience. 1 . 3. PEI-based particles can also be used as adjuvants for vaccines. We determined the optimal DNA:PEI ratios for maximal expression of the reporter gene SEAP while monitoring cytotoxicity following PEI can be used to functionalize single-walled nanotubes (SWNTs) to improve their solubility and biocompatibility while maintaining the structural integrity of the original SWNT. JP JA. Polysciences, Inc. 0 μg/ml and 5. bfrtdb fnpgasod rdix ollpd uvlmh wlcfag qdrc ooejq svs ebwqz lsm xldeg qtppb bai yvzwdn