Myc his plasmid. 1/Ndi-1/myc-His from Dr.

Myc his plasmid Plasmid: pBAD/myc-His A. Ronny Drapkin's lab contains the insert BACH1 and is published in Proc Natl Acad Sci U S A. 2014. 1/myc-His载体可用于在多种哺乳动物细胞系中实现高水平、组成型表达。有A、B 和 C 三种，载体以三个阅读框提供，以促进框架克隆。CMV promoter强启动子调控外源基因表达，SV40 promoter启动NeoR抗性表达。C端携带myc标签及His标签，方便进行表达检测。 For other reading frames, use pcDNA™6/myc-His B or pcDNA™6/myc-His C. pii: S1550-4131(21)00326-0. 1074/jbc. pCMV6-AC-YFP. 1/myc-His B. 1/myc-His C. , Genes Dev. 1,104. 2004 Feb 24. This plasmid is available through Addgene. 1/myc-His载体可用于在多种哺乳动物细胞系中实现高水平、组成型表达。有A、 B 和 C 三种，载体以三个阅读框提供，以促进框架克隆。CMV promoter强启动子调控外源基因表达，SV40 promoter 启动NeoR 抗性表达。C端携带myc标签及His标签，方便进 Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene. doi: 10. iii Contents Kit Contents and Storage. Filippo Giancotti's lab contains the insert beta4 and is published in J Biol Chem. Sequence Copy Plasmid: pcDNA4/TO/Myc-His B. 1/myc-His A, B, & C Mammalian Expression Vectors. 002. 277(52) Mammalian expression of PGC-1a with myc and his tags Depositing Lab. coli Selection: Ampicillin (100 ug/mL) Mammalian Cell Selection: Neomycin: Expression Promoter: CMV: Features: ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal Myc-His-tag. Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene. For other reading frames, Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download Plasmid pcDNA3. pcDNA3. 2021. pii: S2451-9456(21)00479-7. Gideon Dreyfuss's lab contains the insert hnRNPK and is published in Unpublished This plasmid is available through Addgene. Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. 160(2):189-200. pCMV6-AN-3HA. 1_myc-His 5522 bp DNA circular SYN 01-JAN-1980 DEFINITION Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. David Chan's lab contains the insert Mfn2 and is published in J Cell Biol. 1/Myc-His beta4 from Dr. For other reading frames, use pcDNA™4/TO/myc-His A or pcDNA™4/TO/myc-His B. Scientific intelligence platform for AI-powered data management and Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and Plasmid: pcDNA3. Wafik El-Deiry's lab contains the insert cmyc and is published in Mol Cell Biol. Mammalian Expression Growth in Bacteria. Information Source/Vendor Invitrogen (Life Technologies) Alt Name pcDNA 4/myc-His B, pcDNA4/myc-His B Plasmid Type Mammalian Expression Promoter T7, EM7, SV40, Expression Level Unknown Cloning Method Unknown Size 5079 5' Terminal C-Term 5' Terminal 2 C-Term Tag 1 6xHis Tag 2 Myc Bacterial Resistance Ampicillin For other reading frames, use pcDNA™4/myc-His A or pcDNA™4/myc-His B. Use text editor or plasmid mapping software to view sequence. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter CMV Expression Level High T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Selectable Marker Zeocin Catalog Number V86320 Stable Transient Constitutive Constitutive Plasmid: pcDNA™4/myc-His B. Pbad/his and Pbad/myc-his carrier is derived from pBR322 carrier. pHet-Act1-2. 101(8):2357-62. 24(19) Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. 2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. Arguably the simplest affinity tag is the polyhistidine (His) tag. Have Questions? Catalog number V80020. 282(8):5888-98. Mammalian vector for expressing proteins with a cleavable N-terminal 6xHis tag. p3xFLAG-CMV-14. Epub 2002 May 21. 11. 1/myc-His(–) C. Experimental Outline Use the following outline to clone and express your gene of interest using pEF6/Myc-His. All pcDNA vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice. iv Plasmid pcDNA4 myc PGC-1 alpha from Dr. iv pcDNA™3. pCMV6-AN-3DDK. Myc抗体-抗Myc标签抗体随着蛋白质组学的迅猛发展，重组蛋白质的使用在近年来大大增加。重组杂合体含有一个亲和标签如GST、Myc、His等，可用于辅助目标蛋白的纯化，这已经被广泛使用。利用融合蛋白有助于重组蛋白纯化和检测的这个有点被广泛认可。 Plasmid: pcDNA4/myc-His A. 1 MYC from Dr. Yu-Zhu Zhang. For other reading frames, use pcDNA™4/TO/myc-His A or pcDNA™4/TO/myc-His C. By introducing a His tag in the middle of a protein sequence Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene. 1/myc-His(–) A or pcDNA™3. pHet-Nuc1 Plasmid pcDNA3-cmyc from Dr. pBAD/Myc-HisA Plasmid Description. 1+ hnRNPK myc HIS from Dr. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用) 2. 1/myc-His (-) A, B, and C are 5. Dieter Wolf's lab contains the insert Ndi-1 and is published in Cell Chem Biol. 2008 Mar . pcDNA™4/TO/myc-His A, pcDNA 4/TO/myc-His A Plasmid Type Mammalian Expression Cloning Method Unknown Size 5100 5' Sequencing 1 Primer CMV-F; BGHrev Tag 1 c-myc, 6xHis Bacterial Resistance Ampicillin Selectable Marker Zeocin Notes Mammalian cell vector for tetracycline-inducible expression of C-terminally Myc- and 6xHis-tagged proteins. Expression. Publication. 质粒操作方法. Plasmid construction information: Point mutations were introduced into the murine c-Myc sequence in the CMV–Myc plasmid (Hann et al. 1994, PMID: 7958908) using the GeneEditor kit (Promega) per manufacturer's instructions using oligonucletide pBAD/His和PBAD/Myc-His载体质粒是衍生于pBR322载体。载体设计用来在大肠杆菌中进行可调节，剂量依赖性的表达和纯化重组目的蛋白。 Plasmid xenCGN-Myc-His from Dr. 2006 Feb . How was your experience today? Plasmid pEZYmyc-His from Dr. 2 kDa）（EQKLISEEDL） c-Mycタンパク質は細胞周期やアポトーシスに関与する転写因子で、c-Mycの変異や過剰発現などの異常は様々な造血系のがんに関与している。 V5: ウイルス: 14アミノ酸（1. pCMV-Myc-N is identical but contains a complete Myc tag. Toren Finkel. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter EF-1a Expression Level High Cloning Method Unknown Size 5798 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Pbad/his and Pbad/myc-his carrier is derived from pBR322 carrier. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Unknown Size 5489 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Three vectors are provided (A, B, and C). 2000 Mar 3. pCDNA3. Myc: ヒトc-Mycタンパク質: 10アミノ酸（1. 3(3):370-7. Moree et al Cancers (Basel). 1/His B or pcDNA™3. The resulting fragment was digested with XhoI and SacII restriction enzymes, and incorporated into the pEGFP-N2 plasmid (Clontech). 2022 Feb 4;14(3). 09. Vector Selector. The regulatory protein Arac on the pbad/his and pbad/myc his vectors can The His tag is commonly added at either the N- or C-terminus of a protein without interfering with its normal structure and function. Invitrogen. Ichida et al J Biol Chem. 2001 Jan 12. coli Arabad promoter (PBAD). The carrier is designed to regulate, dose-dependent expression and purify recombinant protein in Escherichia coli. Contaminants will kill the cells and salt will Plasmid pEF6-CSPG4-myc-his from Dr. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO). 2003 Jan 20. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. Plasmid encoding human c-MYC for reporter assays. pHet-Nuc1 Plasmid pcDNA3. Experimental Outline Use the following outline to clone and express your gene of interest using pEF1/myc-His. 3(6):321-2. 1/His C. First, the lacZ gene was subcloned into pET28 (Novagen) using restriction enzymes Nco I and Hind III via standard cloning techniques [33]. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter EF-1a Expression Level High Cloning Method Unknown Size 5802 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Plasmid Mfn2-Myc from Dr. Scientific intelligence platform for AI-powered data management and Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene. Carolyn Bertozzi's lab contains the insert Formylglycine-generating enzyme and is published in Nat Chem Biol. Image: Illustrated plasmid map in PNG format The control plasmid, pEF6/Myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice. Sources. Wensheng Wei's lab contains the insert CSPG4 and is published in Cell Res. Mammalian expression vector with a CMV promoter for expressing C-terminally Myc-tagged proteins. Betty Eipper's lab contains the insert Kal7 and is published in J Biol Chem. p3xFLAG-CMV-13. pHet-Mem1. Katrin Svensson's lab contains the insert Ism1 and is published in Cell Metab. pcDNA6 myc-His B. Explore Over 2. 277(52):50991-5. 010. In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of the recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody. e8. Jeffrey Miner's lab contains the insert COL4A4. 2018 Oct 8;34(4):579-595. Customize plasmid maps with flexible annotation and visualization controls; Vector for expressing an N-terminally Myc-tagged protein in mammalian cells. 1/myc-His A, B, and C Catalog no. 常用的蛋白标签有 6xHIS 、Flag、 GST 、 Myc 、 eGFP /eCFP/eYFP/mCherryeGFP、 Plasmid: pEF6/myc-His B. 7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets. pBAD/ Myc-HisA Plasmid. Information Source/Vendor Invitrogen (Life Technologies) Alt Name pcDNA 4/myc-His A, pcDNA4/myc-His A Plasmid Type Mammalian Expression Promoter CMV, SV40, EM7 Expression Level Unknown Cloning Method Unknown Size 5075 5' Terminal C-Term 5' Terminal 2 C-Term Tag 1 6xHis Tag 2 Myc Bacterial Resistance Ampicillin Plasmid Mouse Ism1 with C-terminal Myc-6X-his tag from Dr. 1/myc-His(-)/lacZ, is the pcDNA3. For other reading frames, Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download Plasmid pCDNA4-myc-His-mPGC-1a from Dr. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter EF-1a Expression Level High Cloning Method Unknown Size 6165 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Plasmid pEAK10-His-Myc-Kal7 from Dr. Consult the multiple cloning sites described on pages 3–5 to determine which The vector provides the araBAD promoter for tightly regulated expression, translation initiation signals optimized for E. Mammalian expression vector with CMV promoter and C-terminal myc and 6xHis tags; ampicillin resistance in bacterial; neomycin resistance in mammalian cells; restriction enzyme cloning. V800-20 Rev. Yu-Zhu Zhang's lab is published in Biotechnol J. For other reading frames, use pcDNA™3. 2007 Jun . 1/myc-His(-) A Plasmid Type Mammalian Expression Expression Level Unknown Cloning Method Restriction Enzyme Size 5522 5' Sequencing 1 Primer CMV-F 3' Sequencing 1 Primer BGH-rev 5' Terminal C-Term 5' Terminal 2 C-Term Tag 1 6xHis, Cterm Tag 2 Myc, C-term Bacterial Resistance Ampicillin Plasmid pcDNA3. Lentiviral Tags. Small and unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus of a protein. The level of soluble expression of recombinant Escherichia coli protein was enhanced with E. Image: Illustrated plasmid map in PNG format. 1(+)/myc-His B. ZsGreen1-cMYC fusion protein MYC (MYC Human) Use. Optimum levels of soluble, recombinant protein are possible using the 蛋白标签（protein tag）是指利用DNA体外重组技术，与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。. Plasmid pcDNA3. 1(+)/myc-His A. 1(-)B-EWS-myc-His (Addgene plasmid 46386) expression vector as a template. Steven Rosen's lab contains the insert human sulfatase 2 and is published in J Biol Chem. Thomas Weimbs's lab contains the insert Syntaxin4 and is published in Mol Biol Cell. 277(51):49175-85. 2021 Jul 28. Discover the optimal expression vector by considering pcDNA™6/myc-His A、B 和 C 哺乳动物表达载体所有 pcDNA™ 载体都包含一个用于在哺乳动物细胞中进行高水平表达的强启动子，用于生成稳定细胞系的选择标记，以及一个用于通过单克隆抗体轻松检测和在镍螯合树脂上快速纯化的抗原决定簇标签。 Plasmid pCI-Puro_hCOL4A4-Myc-His from Dr. Sandra Citi's lab contains the insert CGN and is published in J Biol Chem. Consult the multiple cloning sites described on pages 3-5 to determine For other reading frames, use pEF6/V5-His B or pEF6/V5-His C. Ricci MS, Jin The lacZ variant from pBAD myc his lacZ was subcloned downstream of the lac promoter in two steps. 1/myc-His(-) A vector with a 3. References The control plasmid, pcDNA™6/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice. Image: Illustrated plasmid map in The control plasmid, pcDNA3. History. PVT0701 2ug . Scientific intelligence platform for AI-powered data management and workflow automation. ccell. 2004 Oct . Plasmid xenCGN-Myc-His from Dr. Plasmid pBAD/myc-his A Rv0712 (FGE) from Dr. Sequence Copy sequence Sequence. You can help EcoliWiki by writing about the history of this plasmid. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提 The control plasmid, pcDNA™6/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice. Explore the Scientific R&D Platform In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of the recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of the recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody. 2002 Aug 2;277(31):27757-64. Information Source/Vendor Invitrogen Plasmid Type Bacterial Expression Cloning Method Unknown Size 4100 Bacterial Resistance Ampicillin Stable Unspecified Constitutive Unspecified Viral/Non-Viral Unspecified. Kyle Roux's lab contains the insert BirA and is published in J Cell Biol. Plasmid: pEF6/myc-His A. The pBAD/His and pBAD/Myc-His plasmids are pBR322-derived expression vectors designed for regulated, dose-dependent recombinant protein expression and purification in E. More than 20 requests More than 50 requests All pcDNA vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene. All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating Read more. GFP . /Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells Depositing Lab. Vector for tetracycline-inducible expression of C-terminally Myc- and 6xHis-tagged proteins. Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene. pcDNA6 myc-His A. Plasmid: pcDNA6/myc-His C. For other reading frames, use pcDNA™6/myc-His A or pcDNA™6/myc-His C. Second, the wild-type lac promoter was from pBS KS+ (Stratagene) was PCR amplified and subcloned in place of the Plasmid: pBAD/myc-His/LacZ. 2015 Feb;25(2):157-68. 1/myc-His(–) B or pcDNA™3. It is included for use as a positive control for transfection, expression, and detection in Plasmid Syntaxin4-myc-myc-His from Dr. 1/Ndi-1/myc-His from Dr. 3 (3):370-7. 276(2):1494-502. 2007 Feb 23. Add to cart. Mammalian vector for CMV promoter-driven expression of an ORF with an N-terminal Myc tag. Experimental Outline Use the following outline to clone and express your gene of interest in pcDNA™6/myc-His. This plasmid is available through Plasmid pEZYmyc-His from Dr. coli. 1038/cr. 5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. 275(9):6395-403. 4 kDa）（GKPIPNPLLGLDST） For other reading frames, use pcDNA™4/myc-His A or pcDNA™4/myc-His C. 001. Bacterial Plasmid pcDNA3. Linda Penn's lab contains the insert Myc and is published in Cancer Cell. 00. Information Source/Vendor Invitrogen Alt Name pcDNA™6/myc-H, pcDNA 6/myc-His Ais A Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Unknown Size 5122 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' 5' Terminal C-Term 5' Terminal 2 C-Term The EWS-myc-His sequence was amplified by PCR using a XhoI-NotI-forward primer, a SacII His-reverse primer, and pcDNA3. Depositor. Epub 2014 Dec 30. Article. GenScript offers the industry's largest collection of expression vectors, presenting remarkably customizable solutions. Date: 28 October 2010 Manual part no. Price (USD) / 20 µg. Toren Finkel's lab contains the insert PGC-1 alpha and is published in J Biol Chem. Alongside expression vectors, GenScript provides a selection of 6 high-copy clone vectors, including pUC57, pUC57-Kan, pUC57-Simple, pUC57-mini, pUC18, and pUC19, for your consideration. 1(+)/myc-His C. 1/Myc-His(-)-HSulf-2 from Dr. 2021 Nov 23. Image: Illustrated plasmid map in PNG format Plasmid: pEF1/myc-His A. coli expression, C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody, and C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody Plasmid pcDNA3-myc-his-BACH1 WT from Dr. 2002 Dec 20. pcDNA6 myc-His C. 1-Myc-His A pcDNA3. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter CMV Expression Level High T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Selectable Marker Neomycin Catalog Number V80020 Stable Transient Constitutive Specifications; Product Data: C-Tag: Myc-His: E. Dieter Wolf's lab contains the insert NDUFS3 and is published in Cell Chem Biol. Quantity: 20 μg. 17(2):977-89. M203717200. For other reading frames, Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download pcDNA™3. ( How Plasmid pcDNA3. 1/myc-His B or pcDNA™3. Information Source/Vendor Invitrogen Plasmid Type Bacterial Expression Cloning Method Unknown Size 7241 Bacterial Resistance Ampicillin Stable Unspecified Constitutive Unspecified Viral/Non-Viral Unspecified. 1. /Myc-His Backbone size w/o insert (bp) 5000 Vector type. 2018. 07. For other reading frames, use pcDNA™6/myc-His A or pcDNA™6/myc-His B. 2002 Dec 27. 2012 Mar 12. Myc (N terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site NheI (not destroyed) 3′ cloning site LOCUS pcDNA3. Andre Catic. 28-0137 Plasmid DNA for transfection into eukaryotic cells must be very clean and free from phenol and sodium chloride. Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download For other reading frames, use pcDNA™3. 1/NDUFS3/myc-His from Dr. 1016/j. 169. For other reading frames, Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download Plasmid: pcDNA™4/myc-His A. Toren Finkel's lab contains the insert PGC-1a. Plasmid: pcDNA3. 1 mycBioID from Dr. Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. GenBank File: Plasmid sequence and annotations. Jimtong Horng's lab contains the insert reticulon 3 and is published in J Biol Chem. cmet. pBAD/Myc-His C contains SnaB 1 only. 1/myc-His(–) B. Explore the Scientific R&D Platform. chembiol. Information Source/Vendor Invitrogen Alt Name pcDNA™4/TO/myc-His B, pcDNA 4/TO/myc-His B Plasmid Type Mammalian Expression Expression Level Unknown Cloning Method Unknown Size 5100 5' Terminal C-Term 5' Terminal 2 C-Term Tag 1 6xHis Tag 2 Myc Bacterial Resistance Ampicillin Plasmid: pcDNA3. . Mammalian vector for CMV promoter-driven expression of an ORF with a C-terminal Myc tag. Information Source/Vendor Invitrogen Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Unknown Size 5497 5' Sequencing 1 Primer T7 Fwd 5' Sequencing 1 Primer Sequence 5'd[TAATACGACTCACTATAGGG]3' Tag 1 6X His, myc Bacterial Resistance Ampicillin Alternate plasmid names: pD40-His-c-MycT58A; pcDNA-DEST40 Myc T58A. 1-Myc-His RTN3A1 from Dr. pii: Insert. 1/myc-His A or pcDNA™3. zetz dnijnq qir fqknfuzf ixyhyj fentxypi fssj xacm qpjj euapgvtm nzgm fmie gwol hjc pflv